Coding

Part:BBa_K5218007:Design

Designed by: Amy Fu, Juliette, Lichuan Chen, Xiaojuan Wang   Group: iGEM24_BGI-MammothEdu-South   (2024-09-03)


PpC3H


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 690
    Illegal XbaI site found at 369
    Illegal XbaI site found at 573
    Illegal PstI site found at 855
    Illegal PstI site found at 1539
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 690
    Illegal PstI site found at 855
    Illegal PstI site found at 1539
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 690
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 690
    Illegal XbaI site found at 369
    Illegal XbaI site found at 573
    Illegal PstI site found at 855
    Illegal PstI site found at 1539
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 690
    Illegal XbaI site found at 369
    Illegal XbaI site found at 573
    Illegal PstI site found at 855
    Illegal PstI site found at 1539
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 352
    Illegal BsaI.rc site found at 933
    Illegal BsaI.rc site found at 1197


Design Notes

nil


Source

The PpC3H gene loci is LOC112275074, transcript loci is XM_024505055.1 (NCBI). Team BGI-MammothEdu-South 2024 extracted Physcomitrella patensa total RNA, reverse transcribed into cDNA and cloned the PpC3H CDS with specific primer pairs.

References